Characterisation of the <i>Arabidopsis thaliana</i> telomerase TERT-TR complex
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F24%3A00586445" target="_blank" >RIV/68081707:_____/24:00586445 - isvavai.cz</a>
Result on the web
<a href="https://link.springer.com/article/10.1007/s11103-024-01461-w" target="_blank" >https://link.springer.com/article/10.1007/s11103-024-01461-w</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s11103-024-01461-w" target="_blank" >10.1007/s11103-024-01461-w</a>
Alternative languages
Result language
angličtina
Original language name
Characterisation of the <i>Arabidopsis thaliana</i> telomerase TERT-TR complex
Original language description
Most eukaryotic organisms employ a telomerase complex for the maintenance of chromosome ends. The core of this complex is composed of telomerase reverse transcriptase (TERT) and telomerase RNA (TR) subunits. The TERT reverse transcriptase (RT) domain synthesises telomeric DNA using the TR template sequence. The other TERT domains contribute to this process in different ways. In particular, the TERT RNA-binding domain (TRBD) interacts with specific TR motif(s). Using a yeast 3-hybrid system, we show the critical role of Arabidopsis thaliana (At) TRBD and embryophyta-conserved KRxR motif in the unstructured linker preceding the TRBD domain for binding to the recently identified AtTR subunit. We also show the essential role of the predicted P4 stem and pseudoknot AtTR structures and provide evidence for the binding of AtTRBD to pseudoknot and KRxR motif stabilising interaction with the P4 stem structure. Our results thus provide the first insight into the core part of the plant telomerase complex.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10611 - Plant sciences, botany
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Plant Molecular Biology
ISSN
0167-4412
e-ISSN
1573-5028
Volume of the periodical
114
Issue of the periodical within the volume
3
Country of publishing house
DE - GERMANY
Number of pages
12
Pages from-to
56
UT code for WoS article
001222709400002
EID of the result in the Scopus database
2-s2.0-85192957155