Detection of thymine stretches in DNA oligonucleotides at the surface of hanging mercury drop electrode using thymine-mercury base pair formation

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F24%3A00618564" target="_blank" >RIV/68081707:_____/24:00618564 - isvavai.cz</a>
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S0013468624017341?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0013468624017341?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.electacta.2024.145498" target="_blank" >10.1016/j.electacta.2024.145498</a>

Result language
angličtina
Original language name
Detection of thymine stretches in DNA oligonucleotides at the surface of hanging mercury drop electrode using thymine-mercury base pair formation
Original language description
Thymine-mercury-thymine base pair is a well described type of metal ion-mediated interaction in DNA, with a frequent use in construction of biosensors for mercury detection. In this work we took advantage of this interaction to study behavior of thymine-containing oligodeoxynucleotide (ODN) layers adsorbed on the surface of hanging mercury drop electrode (HMDE), which served as a source of mercury cations upon applying a mildly positive potential (+0.15 V). We confirmed formation of the thymine-mercury complex at the surface of HMDE by observing a pair of redox peaks specific for mercury in dependence on presence of the thymine stretches in ODNs. Deoxythymidine mono- or triphosphate gave much weaker signals due to the mercury-thymine complexes. We confirmed that the complex formation can cause unwinding of the AT duplex and concluded that this approach can be used to observe desorption of the homothymine ODN from the HMDE surface. Moreover, we were able to detect stretches of as little as three thymines in ODNs derived from different telomeric repeat sequences.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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