Transcriptome-scale analysis uncovers conserved residues in the hydrophobic core of the bacterial RNA chaperone Hfq required for small regulatory RNA stability

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F25%3A00619092" target="_blank" >RIV/68081707:_____/25:00619092 - isvavai.cz</a>
Result on the web
<a href="https://academic.oup.com/nar/article/53/3/gkaf019/7983884?login=true" target="_blank" >https://academic.oup.com/nar/article/53/3/gkaf019/7983884?login=true</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1093/nar/gkaf019" target="_blank" >10.1093/nar/gkaf019</a>

Result language
angličtina
Original language name
Transcriptome-scale analysis uncovers conserved residues in the hydrophobic core of the bacterial RNA chaperone Hfq required for small regulatory RNA stability
Original language description
The Hfq protein, functioning as an RNA chaperone, plays a key role in the regulation of gene expression in bacteria and significantly facilitates the action of small regulatory RNAs (sRNAs). In our study, we created a systematic library of Hfq mutations, where individual amino acids were replaced by alanine, and these mutants were tested experimentally and using molecular dynamics methods. The results confirmed the key role of three RNA-binding surfaces for Hfq function. We discovered two previously undescribed, evolutionarily conserved amino acids – V22 and G34 – in the hydrophobic core of Hfq, which have a crucial impact on its ability to bind RNA inside the bacterial cell. Transcriptome analysis showed that the V22A and G34A mutations cause extensive destabilization of sRNA, to the same extent as in bacteria completely lacking Hfq. Nevertheless, these mutations led to only a slight change in the stability and structure of Hfq.
Czech name
—
Czech description
—

Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10608 - Biochemistry and molecular biology

Project
<a href="/en/project/GA23-05639S" target="_blank" >GA23-05639S: Molecular dynamics simulations of RNA: from static structures to molecular ensembles</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Similar results(10)