A biotin-HaloTag ligand enables efficient affinity capture of protein variants from live cells

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F25%3A00637714" target="_blank" >RIV/68081707:_____/25:00637714 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14310/25:00142954 RIV/00159816:_____/25:00082440
Result on the web
<a href="https://rupress.org/jcb/article-abstract/224/8/e202410025/278122/A-biotin-HaloTag-ligand-enables-efficient-affinity?redirectedFrom=fulltext" target="_blank" >https://rupress.org/jcb/article-abstract/224/8/e202410025/278122/A-biotin-HaloTag-ligand-enables-efficient-affinity?redirectedFrom=fulltext</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1083/jcb.202410025" target="_blank" >10.1083/jcb.202410025</a>

Result language
angličtina
Original language name
A biotin-HaloTag ligand enables efficient affinity capture of protein variants from live cells
Original language description
HaloTag technology represents a versatile tool for studying proteins. Fluorescent HaloTag ligands employed in sequential labeling led to the discovery of distinct protein variants for histones, cohesins, and MCM complexes. However, an efficient biochemical approach to separate these distinct protein variants to study their biological functions is missing. Principally, being a gap in technology, the HaloTag toolbox lacks affinity ligands displaying good cell permeability and efficient affinity capture. Here, we describe the design, synthesis, and validation of a new cell-permeable biotin-HaloTag ligand, which allows rapid labeling of Halo-tagged proteins in live cells and their efficient separation using streptavidin pull-down. We provide a proof-of-concept application of how to use the herein-developed affinity ligand in sequential labeling to biochemically separate protein variants and study their biological properties. This approach enables to address fundamental questions concerning essential cellular processes, including genome duplication and chromatin maintenance.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10601 - Cell biology

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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