Standards for capillary isoelectric focusing with laser induced fluorescence detection.
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F01%3A25010051" target="_blank" >RIV/68081715:_____/01:25010051 - isvavai.cz</a>
Alternative codes found
RIV/00216305:26310/01:PU22169
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Standards for capillary isoelectric focusing with laser induced fluorescence detection.
Original language description
Proteins, peptides or BGE ampholytes labeled with suitable dyes were previously suggested as pI markers. However, both proteins and background ampholytes have many possible reactive sites for reactive dyes and this might result in formation of heterogeneously labeled product with respect to pI. By contrast, the low molecular pI markers can be prepared with heterogeneity and greater stability than proteins and could potentially be good pI markers as found in case of UV detection. To improve the laser induced fluorescence detection, new low molecular fluorescent compounds excitable around 490 nm with suitable acidobasic and electrophoretic properties were prepared and focused in capillary IEF with photometric or fluorometric detection. The experimental setup of IEF and properties of new laser induced fluorescent pI markers are given.
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Result continuities
Project
<a href="/en/project/IAA4031901" target="_blank" >IAA4031901: Capillary isotachoforesis with combined continuous conductivity and pH gradients</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2001
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
2nd International Symposium Separations in the BioSciences. SBS 2001.
ISBN
80-7080-437-8
ISSN
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e-ISSN
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Number of pages
1
Pages from-to
81
Publisher name
N/A
Place of publication
Praha
Event location
Praha [CZ]
Event date
Sep 17, 2001
Type of event by nationality
EUR - Evropská akce
UT code for WoS article
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