Identification of barley proteins from in-gel digests by MALDI-TOF MS.

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F01%3A25010057" target="_blank" >RIV/68081715:_____/01:25010057 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Identification of barley proteins from in-gel digests by MALDI-TOF MS.
Original language description
Proteins were digested from barley leaves, then separated using ID gel electrophoresis and in-gel digested with trypsin. Peptide masses were adjusted through MALDI-TOF and then put into the database searching program. This method is not always successfulbecause the success depends mainly on presence of the protein in the database. In the case of barley only about 20 of genome is known and moreover there exist many variants of barley. In spite of this complications, several proteins were identified. Itproved that the combination of in-gel digestion and MALDI-TOF is suitable for a first attempt of identification of unknown proteins.
Czech name
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Czech description
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2001
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
Proceedings of the 19th Informal Meeting on Mass Spectrometry.
ISBN
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ISSN
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e-ISSN
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Number of pages
1
Pages from-to
136
Publisher name
Hungarian Academy of Sciences
Place of publication
Budapest
Event location
Noszvaj [HU]
Event date
Apr 29, 2001
Type of event by nationality
EUR - Evropská akce
UT code for WoS article
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