Preparative divergent flow IEF without carrier ampholytes for separation of complex biological samples
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F10%3A00338379" target="_blank" >RIV/68081715:_____/10:00338379 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Preparative divergent flow IEF without carrier ampholytes for separation of complex biological samples
Original language description
Based on the principles of recently newly established electrophoretic method called divergent flow IEF (DF IEF), we have tested the DF IEF instrument operating without the use of background carrier ampholytes. In DF IEF, the proteins are separated, desalted and concentrated in one step. We have shown that during separation and focusing of sample consisting of high numbers of proteins (yeast lysate and wheat flour extract), the pH gradient of preparative divergent flow IEF (DF IEF) can be created by autofocusing of the sample components themselves.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Result continuities
Project
<a href="/en/project/IAAX00310701" target="_blank" >IAAX00310701: Rapid detection and identification of pathogenic microorganisms and viruses by electromigration techniques and mass spectrometry</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2010
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Electrophoresis
ISSN
0173-0835
e-ISSN
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Volume of the periodical
31
Issue of the periodical within the volume
3
Country of publishing house
DE - GERMANY
Number of pages
7
Pages from-to
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UT code for WoS article
000274603200001
EID of the result in the Scopus database
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