Oriented immobilization of PNgase F on a porous polymer monolith for rapid N-glycan release

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F12%3A00386014" target="_blank" >RIV/68081715:_____/12:00386014 - isvavai.cz</a>
Result on the web
<a href="http://hdl.handle.net/11104/0215253" target="_blank" >http://hdl.handle.net/11104/0215253</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Oriented immobilization of PNgase F on a porous polymer monolith for rapid N-glycan release
Original language description
We demonstrate a simple and rapid method for the oriented immobilization of peptide-N4-(Nacetyl- glucosaminyl) asparagine amidase F (PNGase F) on a porous polymer monolith. The oriented immobilization is based on the affinity of glutathione-S-transferase(GST) tagged PNGase F towards glutathione modified monolith prepared in the capillary format. This approach allows the oriented and easily replaceable immobilization of PNGase F for rapid and efficient release of N-linked glycans. The reactor was testedby deglycosylation of several native glycoproteins such as ribonuclease B, fetuin or human immunoglobulin G. The proteins were effectively deglycosylated in several minutes and the released N-linked glycans were analyzed using off-line MALDI/MS or CE/LIF.
Czech name
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Czech description
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Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
CECE 2012. 9th International Interdisciplinary Meeting on Bioanalysis
ISBN
978-80-904959-1-3
ISSN
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e-ISSN
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Number of pages
3
Pages from-to
36-38
Publisher name
Ústav analytické chemie AV ČR, v. v. i
Place of publication
Brno
Event location
Brno
Event date
Nov 1, 2012
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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