Micropreparative solution isoelectric focusing of peptides and proteins in nonwoven strip
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F12%3A00386015" target="_blank" >RIV/68081715:_____/12:00386015 - isvavai.cz</a>
Result on the web
<a href="http://hdl.handle.net/11104/0215261" target="_blank" >http://hdl.handle.net/11104/0215261</a>
DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Micropreparative solution isoelectric focusing of peptides and proteins in nonwoven strip
Original language description
Recently we devised a new instrument for micropreparative analysis [1]. It is based on solution phase isoelectric focusing (sIEF) performed in a narrow channel (approximately 2 ? 4 mm wide) with a strip of nonwoven fabric serving as the analysis bed. Thestrip can be precut before an analysis to make harvesting of fractions easier. Isoelectric focusing is driven by a programmable electrophoretic power supply. Progress of analysis is monitored by addition of colored isoelectric point (pI) markers. Usually sIEF runs are performed overnight. Evaporation of water is one of crucial features of analysis and it forces free liquid to shrink into the nonwoven fabric strip. Moreover, water evaporation increases liquid viscosity by altering ethylene glycol/waterratio. Fractions can be harvested simply by collecting and washing precut segments after the sIEF run. In this paper we used sIEF device for purification of caseinomacropeptide (CMP) from crude whey. Obtained fractions were further analyz
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
CECE 2012. 9th International Interdisciplinary Meeting on Bioanalysis
ISBN
978-80-904959-1-3
ISSN
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e-ISSN
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Number of pages
3
Pages from-to
66-68
Publisher name
Ústav analytické chemie AV ČR, v. v. i
Place of publication
Brno
Event location
Brno
Event date
Nov 1, 2012
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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