pI-Control in comparative fluorescence gel electrophoresis (CoFGE) using amphoteric azo dyes

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F15%3A00447122" target="_blank" >RIV/68081715:_____/15:00447122 - isvavai.cz</a>
Result on the web
<a href="http://ac.els-cdn.com/S2212968515000094/1-s2.0-S2212968515000094-main.pdf?_tid=7c92fa40-56e6-11e5-b36a-00000aab0f01&acdnat=1441798543_19612c0d7466780944bc4ae22173da92" target="_blank" >http://ac.els-cdn.com/S2212968515000094/1-s2.0-S2212968515000094-main.pdf?_tid=7c92fa40-56e6-11e5-b36a-00000aab0f01&acdnat=1441798543_19612c0d7466780944bc4ae22173da92</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.euprot.2015.03.003" target="_blank" >10.1016/j.euprot.2015.03.003</a>

Result language
angličtina
Original language name
pI-Control in comparative fluorescence gel electrophoresis (CoFGE) using amphoteric azo dyes
Original language description
Gel-to-gel variation is a major problem in two-dimensional (2D) gel electrophoresis. Vertical and horizontal comparative fluorescence gel electrophoresis (v/hCoFGE) were recently introduced to improve the reproducibility of protein spot assignment. There, in a combination of 1D- and 2D-PAGE, protein analytes were overlaid with a reference grid which allowed correcting for variation in the y-direction. However, control of the first dimension, pI (x-direction), was missing so far. Here, we applied amphoteric azo dyes for this purpose completing the CoFGE toolkit.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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