SDS-PAGE and gel IEF – tool for differentiation of methicillin-resistant and methicillin-sensitive strains of Staphylococcus aureus

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F16%3A00449521" target="_blank" >RIV/68081715:_____/16:00449521 - isvavai.cz</a>

Alternative codes found

RIV/00159816:_____/16:00064261 RIV/00216224:14110/16:00087691

Result on the web

<a href="http://hdl.handle.net/11104/0251069" target="_blank" >http://hdl.handle.net/11104/0251069</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00284-015-0939-y" target="_blank" >10.1007/s00284-015-0939-y</a>

Result language

angličtina

Original language name

SDS-PAGE and gel IEF – tool for differentiation of methicillin-resistant and methicillin-sensitive strains of Staphylococcus aureus

Original language description

The methicillin-resistant Staphylococcus aureus causes difficult-to-treat healthcare-associated infections in humans. For fast and effective selection of an appropriate antibiotic therapy, it is essential to have rapid and reliable methods for differentiation of methicillin-resistant Staphylococcus aureus from less dangerous methicillin-sensitive Staphylococcus aureus. There have been many methods for the identification of methicillin-resistant Staphylococcus aureus described but none has been accepted as an international standard. The most commonly used techniques such as phenotyping and genotyping have a few disadvantages, for instance, these techniques are not reproducible and stable. In addition, they are time-consuming, expensive, and they are not capable to distinguish all Staphylococcus aureus strains. In this study, the methicillin-resistant and methicillin-sensitive Staphylococcus aureus isolates obtained from patients were extracted in hot water. The released proteins were characterized by sodium dodecylsulphate polyacrylamide gel electrophoresis and gel isoelectric focusing. These two methods were able to differentiate among tested bacterial strains. The proposed methods are time saving, they are applicable in standard biochemical laboratories, and they do not require any expensive equipment.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10406 - Analytical chemistry

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Current Microbiology

ISSN

0343-8651

e-ISSN

—

Volume of the periodical

72

Issue of the periodical within the volume

3

Country of publishing house

US - UNITED STATES

Number of pages

6

Pages from-to

315-320

UT code for WoS article

000371251200011

EID of the result in the Scopus database

2-s2.0-84957870159