Bridged polysilsesquioxane-based wide-bore monolithic capillary columns for hydrophilic interaction chromatography

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F17%3A00469740" target="_blank" >RIV/68081715:_____/17:00469740 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.chroma.2016.11.062" target="_blank" >http://dx.doi.org/10.1016/j.chroma.2016.11.062</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.chroma.2016.11.062" target="_blank" >10.1016/j.chroma.2016.11.062</a>

Result language
angličtina
Original language name
Bridged polysilsesquioxane-based wide-bore monolithic capillary columns for hydrophilic interaction chromatography
Original language description
The synthesis and characterization of large-bore silica-based monolithic capillary columns (0.32 mm × 150 mm) are presented. Columns were prepared by acidic hydrolysis of a mixture containing tetramethoxysilane (TMOS) and 1,2-bis(trimethoxysilyl)ethane (BTME) in different molar ratios in the presence of polyethylene glycol and urea. The monoliths were modified by zwitterionic monomer [2-(methacryloyloxy)ethyl]-dimethyl-(3-sulfopropyl)-ammonium hydroxide via 3-(trimethoxysilyl)propyl methacrylate. Prepared stationary phases were evaluated by scanning electron microscopy and chromatographic separation of nucleobases and their derivatives in the HILIC mode. The best chromatographic results were obtained with the column prepared from the reaction mixture containing BTME and TMOS in a 1:4 molar ratio. The permeability of such column reached 1.68 × 10−14 m2 and the efficiency, expressed as a height equivalent of the theoretical plate, did not exceed 10.5 μm for the tested compounds. The columns were successfully applied to HILIC separation of native and labeled oligosaccharides and glycans released from bovine ribonuclease B and human immunoglobulin G.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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