Multi-fractional analysis of molecular diffusion in polymer multilayers by FRAP: A new simulation-based approach

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F18%3A00486843" target="_blank" >RIV/68081715:_____/18:00486843 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1021/acs.jpcb.7b11051" target="_blank" >http://dx.doi.org/10.1021/acs.jpcb.7b11051</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1021/acs.jpcb.7b11051" target="_blank" >10.1021/acs.jpcb.7b11051</a>

Result language

angličtina

Original language name

Multi-fractional analysis of molecular diffusion in polymer multilayers by FRAP: A new simulation-based approach

Original language description

Here we present a novel simulation optimization-based approach (S-approach) that significantly broadens possibilities of the analysis. In the S-approach, possible fluorescence recovery scenarios are primarily simulated and afterward compared with a real measurement while optimizing parameters of a model until a sufficient match is achieved. This makes it possible to reveal multifractional molecular diffusion. Fluorescent latex particles of different size and fluorescein isothiocyanate in an aqueous medium were utilized as test systems. Finally, the S-approach has been used to evaluate diffusion of cytochrome c loaded into multilayers made of hyaluronan and polylysine. Software for evaluation of multifractional molecular diffusion by S-approach has been developed aiming to offer maximal versatility and user-friendly way for analysis.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10406 - Analytical chemistry

Project

<a href="/en/project/GBP206%2F12%2FG014" target="_blank" >GBP206/12/G014: Center for advanced bioanalytical technologies</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Physical Chemistry B

ISSN

1520-6106

e-ISSN

—

Volume of the periodical

122

Issue of the periodical within the volume

3

Country of publishing house

US - UNITED STATES

Number of pages

11

Pages from-to

1323-1333

UT code for WoS article

000423652900035

EID of the result in the Scopus database

2-s2.0-85041171903