Determination of the main phase transition temperature of phospholipids by nanoplasmonic sensing

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F18%3A00494894" target="_blank" >RIV/68081715:_____/18:00494894 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1038/s41598-018-33107-5" target="_blank" >http://dx.doi.org/10.1038/s41598-018-33107-5</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41598-018-33107-5" target="_blank" >10.1038/s41598-018-33107-5</a>

Result language

angličtina

Original language name

Determination of the main phase transition temperature of phospholipids by nanoplasmonic sensing

Original language description

Our study demonstrates that nanoplasmonic sensing (NPS) can be utilized for the determination of the phase transition temperature (T-m) of phospholipids. During the phase transition, the lipid bilayer undergoes a conformational change. Therefore, it is presumed that the T-m of phospholipids can be determined by detecting conformational changes in liposomes. The studied lipids included 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC), and 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC). Liposomes in gel phase are immobilized onto silicon dioxide sensors and the sensor cell temperature is increased until passing the T-m of the lipid. The results show that, when the system temperature approaches the T-m, a drop of the NPS signal is observed. The breakpoints in the temperatures are 22.5 degrees C, 41.0 degrees C, and 55.5 degrees C for DMPC, DPPC, and DSPC, respectively. These values are very close to the theoretical T-m values, i.e., 24 degrees C, 41.4 degrees C, and 55 degrees C for DMPC, DPPC, and DSPC, respectively. Our studies prove that the NPS methodology is a simple and valuable tool for the determination of the T-m of phospholipids.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

21002 - Nano-processes (applications on nano-scale); (biomaterials to be 2.9)

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Scientific Reports

ISSN

2045-2322

e-ISSN

—

Volume of the periodical

8

Issue of the periodical within the volume

OCT

Country of publishing house

GB - UNITED KINGDOM

Number of pages

11

Pages from-to

—

UT code for WoS article

000446325500027

EID of the result in the Scopus database

2-s2.0-85054446384