Preparative and capillary isoelectric focusing for detection and identification of Aspergillus conidia in complex sample matrices

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F18%3A00496008" target="_blank" >RIV/68081715:_____/18:00496008 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14110/18:00106898

Result on the web

<a href="http://dx.doi.org/10.1002/jssc.201800547" target="_blank" >http://dx.doi.org/10.1002/jssc.201800547</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jssc.201800547" target="_blank" >10.1002/jssc.201800547</a>

Result language

angličtina

Original language name

Preparative and capillary isoelectric focusing for detection and identification of Aspergillus conidia in complex sample matrices

Original language description

This study describes a new method for fast identification of highly hydrophobic conidia of Aspergillus species from both simple and complex matrices. The method is based on recently developed preparative isoelectric focusing in a cellulose-based separation medium which had to be modified with respect to the highly hydrophobic surface of the conidia. Although Aspergillus conidia are colored, their zones in the cellulose bed were indicated by colored isoelectric point markers. The isoelectric point values of Aspergillus conidia were determined by capillary isoelectric focusing. Preparative isoelectric focusing was successfully used for preconcentration of individual conidia of cultivated strains of Aspergillus niger, Aspergillus fumigatus, Aspergillus flavus, and Aspergillus parasiticus, and also for separation of the conidia in a mixture. Subsequently, red pepper powder and peanuts spiked with Aspergillus niger and Aspergillus flavus conidia, respectively, were used as complex matrices. The detection limit for identification of the conidia in these complex matrices is 104 conidia mL−1. The presence of conidia in the focused zones was confirmed by their subsequent analysis by capillary isoelectric focusing. Their viability was confirmed by a cultivation of the conidia extracted from the collected fractions after preparative isoelectric focusing

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10406 - Analytical chemistry

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Separation Science

ISSN

1615-9306

e-ISSN

—

Volume of the periodical

41

Issue of the periodical within the volume

22

Country of publishing house

DE - GERMANY

Number of pages

9

Pages from-to

4203-4211

UT code for WoS article

000450408300013

EID of the result in the Scopus database

2-s2.0-85053707407