Optimization of background electrolyte composition for simultaneous contactless conductivity and fluorescence detection in capillary electrophoresis of biological samples

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F19%3A00508882" target="_blank" >RIV/68081715:_____/19:00508882 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14740/19:00108517

Result on the web

<a href="http://hdl.handle.net/11104/0299680" target="_blank" >http://hdl.handle.net/11104/0299680</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/elps.201900112" target="_blank" >10.1002/elps.201900112</a>

Result language

angličtina

Original language name

Optimization of background electrolyte composition for simultaneous contactless conductivity and fluorescence detection in capillary electrophoresis of biological samples

Original language description

In this article, optimization of BGE for simultaneous separation of inorganic ions, organic acids, and glutathione using dual C4D-LIF detection in capillary electrophoresis is presented. The optimized BGE consisted of 30 mM 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid, 15 mM 2-amino-2-hydroxymethyl-propane-1,3-diol, and 2 mM 18-crown-6 at pH 7.2 and allowed simultaneous separation of ten inorganic anions and cations, three organic acids and glutathione in 20 min. The samples were injected hydrodynamically from both capillary ends using the double-opposite end injection principle. Sensitive detection of anions, cations, and organic acids with micromolar LODs using C4D and simultaneously glutathione with nanomolar LODs using LIF was achieved in a single run. The developed BGEmay be useful in analyses of biological samples containing analytes with differing concentrations of several orders of magnitude that is not possible with single detection mode.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10406 - Analytical chemistry

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Electrophoresis

ISSN

0173-0835

e-ISSN

—

Volume of the periodical

40

Issue of the periodical within the volume

18-19

Country of publishing house

DE - GERMANY

Number of pages

8

Pages from-to

2390-2397

UT code for WoS article

000483858400018

EID of the result in the Scopus database

2-s2.0-85068158096