Photon-Upconversion Barcoding with Multiple Barcode Channels: Application for Droplet Microfluidics
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F19%3A00509591" target="_blank" >RIV/68081715:_____/19:00509591 - isvavai.cz</a>
Result on the web
<a href="http://hdl.handle.net/11104/0300289" target="_blank" >http://hdl.handle.net/11104/0300289</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/acs.analchem.9b0311" target="_blank" >10.1021/acs.analchem.9b0311</a>
Alternative languages
Result language
angličtina
Original language name
Photon-Upconversion Barcoding with Multiple Barcode Channels: Application for Droplet Microfluidics
Original language description
Barcoding facilitates high-throughput analytical methods in complex matrixes with a reduced volume of sample, reagents, time, and cost. Because of orthogonality to fluorescence, photon-upconversion barcodes attracted considerable attention in recent years. We constructed an epiluminescence detector, which, for the first time, demonstrated the reading of photon-upconversion spectra from microdroplets in a microfluidic chip with frequency up to 10 Hz. Non-negative least-squares deconvolution enabled the reading of an unprecedented number of photon-upconversion barcode channels (six) from emission spectra (excitation 980 nm, emission 430-875 nm). The standard deviation of barcode reading from microdroplets was ∼1%. Described barcoding can be, for example, used for multiparameter titrations, multiplexed biological and chemical assays, optimizations on a microfluidic platform, and preparation of barcoded concentration gradients and libraries.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
<a href="/en/project/GJ18-03367Y" target="_blank" >GJ18-03367Y: Photon upconversion barcoding for bioanalysis in droplet microfluidics</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Analytical Chemistry
ISSN
0003-2700
e-ISSN
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Volume of the periodical
91
Issue of the periodical within the volume
20
Country of publishing house
US - UNITED STATES
Number of pages
6
Pages from-to
12630-12635
UT code for WoS article
000491219900007
EID of the result in the Scopus database
2-s2.0-85072983372