Fast and highly sensitive laser scanner for recording photon-upconversion luminiscence from planar surfaces

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F20%3A00511604" target="_blank" >RIV/68081715:_____/20:00511604 - isvavai.cz</a>

Result on the web

<a href="https://www.confer.cz/nanocon/2019" target="_blank" >https://www.confer.cz/nanocon/2019</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.37904/nanocon.2019.8563" target="_blank" >10.37904/nanocon.2019.8563</a>

Result language

angličtina

Original language name

Fast and highly sensitive laser scanner for recording photon-upconversion luminiscence from planar surfaces

Original language description

Photon-upconversion nanoparticles (UCNPs) are lanthanide-doped nanocrystals that can be excited by nearinfrared light and emit photon-upconversion luminescence of shorter wavelengths. Advantages of UCNPs include near-infrared excitation, multiple and narrow emission bands, negligible autofluorescence and high stability, which make UCNPs ideal luminescence label for use in biological and chemical assays. These assays - e.g. upconversion-linked immunosorbent assay, western blot, lateral flow assay, gel electrophoresis, thin layer chromatography - commonly require the scanning of a planar surface with a high spatial resolution and an excellent sensitivity. The availability of commercial equipment is recently limited because of the novelty of the photon-upconversion phenomenon. Therefore, we report on the construction of photon-upconversion laser scanner. The scanner consists of a laser scanning head, which is attached to a xy-moving stage. The scanning head itself is constructed as an epiluminescence detector with excitation wavelength of 976 nm. A CCD array spectroscope is connected to the laser head and serves as a sensitive detector of photon-upconversion luminescence. The scanner possesses a spatial resolution of 200 μm, the scanning rate is up to 57 points per second and the sensitivity reaches down to single photon-upconversion nanoparticle.

Czech name

—

Czech description

—

Type

D - Article in proceedings

CEP classification

—

OECD FORD branch

10406 - Analytical chemistry

Project

<a href="/en/project/GJ18-03367Y" target="_blank" >GJ18-03367Y: Photon upconversion barcoding for bioanalysis in droplet microfluidics</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

NANOCON Conference Proceedings - International Conference on Nanomaterials

ISBN

978-808729495-6

ISSN

2694-930X

e-ISSN

—

Number of pages

5

Pages from-to

317-322

Publisher name

Tanger Ltd.

Place of publication

Ostrava

Event location

Brno

Event date

Oct 16, 2019

Type of event by nationality

EUR - Evropská akce

UT code for WoS article

000664115400052