Comparison of oligosaccharide labeling employing reductive amination and hydrazone formation chemistries
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F20%3A00522452" target="_blank" >RIV/68081715:_____/20:00522452 - isvavai.cz</a>
Result on the web
<a href="http://hdl.handle.net/11104/0306941" target="_blank" >http://hdl.handle.net/11104/0306941</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/elps.201900475" target="_blank" >10.1002/elps.201900475</a>
Alternative languages
Result language
angličtina
Original language name
Comparison of oligosaccharide labeling employing reductive amination and hydrazone formation chemistries
Original language description
In this work, we compare labeling by two negatively charged fluorescent labels, 8-aminopyrene-1,3,6-trisulfonic acid (APTS) and 8-(2-hydrazino-2-oxoethoxy)pyrene-1,3,6-ntrisulfonic acid (Cascade Blue hydrazide [CBH]). Effectiveness of the labeling chemistries were investigated by 4-hydroxybenzaldehyde and maltoheptaose followed by LC/UV-MSnand CE/LIF analysis, respectively. The reaction yield of APTS labeling was determined to be only ∼10%. This is due to reduction of almost 90% of the analyte by sodium cyanoborohydridento alcohol, which cannot be further labeled via reductive amination. However, the CBH labeling provides ∼90% reaction yield based on the LC/UV-MS measurements. Thensignificantly higher labeling yield was also confirmed by CE/LIF measurements. Finally, the more effective hydrazone formation technique of CBH was characterized and appliednfor N-linked glycan analysis by CE/LIF.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
<a href="/en/project/GA18-00062S" target="_blank" >GA18-00062S: Highly sensitive glycoprotein analysis with new tagging reagents for fluorescence detection and electrospray ionization mass spectrometry</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Electrophoresis
ISSN
0173-0835
e-ISSN
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Volume of the periodical
41
Issue of the periodical within the volume
9
Country of publishing house
DE - GERMANY
Number of pages
7
Pages from-to
684-690
UT code for WoS article
000513664800001
EID of the result in the Scopus database
2-s2.0-85079716358