DNA damage induced by occupational exposure to copper oxide nanoparticles

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F20%3A00532785" target="_blank" >RIV/68081715:_____/20:00532785 - isvavai.cz</a>

Alternative codes found

RIV/68378041:_____/20:00532785 RIV/00064165:_____/20:10425181 RIV/00216208:11110/20:10425181

Result on the web

<a href="https://www.confer.cz/nanocon/2019" target="_blank" >https://www.confer.cz/nanocon/2019</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.37904/nanocon.2019.8653" target="_blank" >10.37904/nanocon.2019.8653</a>

Result language

angličtina

Original language name

DNA damage induced by occupational exposure to copper oxide nanoparticles

Original language description

Copper oxide nanoparticles (CuO NPs) have a widespread use in industry, chemistry, in production of electronic devices and as an antimicrobial agent. Although copper is an important biogenic element, CuO NPs are toxic with the ability to induce oxidative stress, apoptosis, cell cycle arrest or DNA damage. For humans, the inhalation route is the most common way of exposure to CuO NPs. In the body, CuO NPs may be either deposited in the lungs, or transported to other organs. Their presence usually causes oxidative stress or inflammatory responses, consequently leading to DNA damage. In this study, we investigated the effect of CuO NPs inhalation on DNA damage in a group of researches conducting animal exposure experiments. The subjects were exposed to various metal oxide nanoparticles, including CuO NPs, by inhalation for an average of 4.9 ± 0.4 years. The average mass concentration of Cu in the air during the experiment was 7.3 ± 3.2 ng/m3. Subjects not exposed to nanoparticles served as a control group. We applied micronucleus assay using Human Pan Centromeric probes to detect DNA damage and to distinguish between the frequency of centromere positive (CEN+) and centromere negative (CEN−) micronuclei (MN) in the binucleated cells. Wendid not find differences between both groups for either mean MN frequency (10.38 ± 2.50 vs. 11.88 ± 3.01 MN/1000 binucleated cells), or CEN+/CEN- ratio (58%/42% vs. 55%/45%), for the exposed and controls, respectively. In conclusion, inhalation of CuO NPs at this low-level exposure had no effect on chromosomal losses and/or breaks.

Czech name

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Czech description

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Type

D - Article in proceedings

CEP classification

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OECD FORD branch

10406 - Analytical chemistry

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

NANOCON Conference Proceedings - International Conference on Nanomaterials

ISBN

978-808729495-6

ISSN

2694-930X

e-ISSN

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Number of pages

5

Pages from-to

486-490

Publisher name

Tanger Ltd.

Place of publication

Ostrava

Event location

Brno

Event date

Oct 16, 2019

Type of event by nationality

EUR - Evropská akce

UT code for WoS article

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