Upconversion-Linked Immunoassay for the Diagnosis of Honeybee Disease American Foulbrood
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F21%3A00541721" target="_blank" >RIV/68081715:_____/21:00541721 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14310/21:00121176
Result on the web
<a href="http://hdl.handle.net/11104/0319251" target="_blank" >http://hdl.handle.net/11104/0319251</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1109/JSTQE.2021.3049689" target="_blank" >10.1109/JSTQE.2021.3049689</a>
Alternative languages
Result language
angličtina
Original language name
Upconversion-Linked Immunoassay for the Diagnosis of Honeybee Disease American Foulbrood
Original language description
American foulbrood (AFB) caused by the bacterium Paenibacillus larvae is the most destructive disease of the honeybee brood. Therefore, rapid and sensitive detection methods are required to limit spreading of this pathogen, which has a major impact on agriculture and biodiversity. While P. larvae is typically detected by microbial cultivation or polymerase chain reaction, antibody-based detection represents a viable alternative. Here, we prepared an antibody specific for P. larvae and used it for the development of an upconversion-linked immunosorbent assay (ULISA). Photon-upconversion nanoparticles (UCNP) were conjugated to streptavidin via a PEG-linker using copper-catalyzed click chemistry to replace the enzyme label in conventional enzyme-linked immunosorbent assay (ELISA). The ULISA showed low cross-reactivity and provided a limit of detection of 2.9 x 10(3) CFU/mL, representing a 22-fold improvement compared to the ELISA. This level is within the bacterial loads present in honeybee larvae during an AFB infection. The assay was successfully applied to the analysis of spiked samples of bees, larvae, and hive debris.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
IEEE Journal of Selected Topics in Quantum Electronics
ISSN
1077-260X
e-ISSN
1558-4542
Volume of the periodical
27
Issue of the periodical within the volume
5
Country of publishing house
US - UNITED STATES
Number of pages
11
Pages from-to
6900311
UT code for WoS article
000630043400002
EID of the result in the Scopus database
2-s2.0-85099206204