Six-week inhalation of lead oxide nanoparticles in mice affects antioxidant defense, immune response, kidneys, intestine and bones
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F22%3A00552108" target="_blank" >RIV/68081715:_____/22:00552108 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11110/22:10437012 RIV/00216224:14310/22:00125468
Result on the web
<a href="http://hdl.handle.net/11104/0327254" target="_blank" >http://hdl.handle.net/11104/0327254</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1039/d1en00957e" target="_blank" >10.1039/d1en00957e</a>
Alternative languages
Result language
angličtina
Original language name
Six-week inhalation of lead oxide nanoparticles in mice affects antioxidant defense, immune response, kidneys, intestine and bones
Original language description
Due to widespread use in various industrial processes, the risk of inhalation exposure to lead oxide nanoparticles (PbO NPs) is growing and limited information evokes the need for assessment of hazard to human health. The aim of our work was to evaluate the effects of PbO NPs on blood, immune response, kidneys, intestine, and bones in mice exposed to 121.7 mu g PbO per m(3) continuously for 6 weeks. Immune assays showed significantly increased phagocytic activity of granulocytes, stimulated proliferative activity of spleen lymphocytes, a higher percentage of spleen CD3(-)CD335(+) and thymus CD3(+)CD8(+) cells, and moderately increased proinflammatory cytokines in the PbO NP-exposed group. Analysis of blood antioxidant defense revealed a significantly lower GSH concentration and a decrease in the GSH/GSSG ratio due to PbO NP inhalation. Markers of oxidative stress (AGEs and AOPP) in the intestine, but not in the kidneys, were significantly increased. In the kidney of PbO NP-treated mice, we found elevated expression of proinflammatory factors (TNF-alpha, IL-6, iNOS, and Xdh) and IL-10 and upregulated expression of F4/80 (mature macrophage marker). Moreover, increased expression of pro-fibrotic markers (TGF beta 2, type I and type III collagen) was detected. Massive accumulation of Pb together with a significant decrease of Ca, P, and Mg concentrations in the tibia were accompanied by a nonsignificant increase in bone mineral density. Our findings indicate adverse effects of sub-chronic inhalation of PbO NPs, manifested as undesired stimulation of the immune system, oxidative stress in the intestine, inflammation, and pro-fibrotic response in the kidneys and massive accumulation of Pb in bones.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Environmental Science-Nano
ISSN
2051-8153
e-ISSN
2051-8161
Volume of the periodical
9
Issue of the periodical within the volume
2
Country of publishing house
GB - UNITED KINGDOM
Number of pages
16
Pages from-to
751-766
UT code for WoS article
000741618800001
EID of the result in the Scopus database
2-s2.0-85125318529