Preparative agarose gel electrophoresis for reducing matrix interferences of organoid cell medium prior to LC-MS analysis of insulin
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F24%3A00584554" target="_blank" >RIV/68081715:_____/24:00584554 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14310/24:00135448
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S0021967324000426?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0021967324000426?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.chroma.2024.464669" target="_blank" >10.1016/j.chroma.2024.464669</a>
Alternative languages
Result language
angličtina
Original language name
Preparative agarose gel electrophoresis for reducing matrix interferences of organoid cell medium prior to LC-MS analysis of insulin
Original language description
Organoids are 3D cell cultures with microanatomies mimicking aspects of real organs, useful for e.g. animal free studies of development, disease, and drug discovery. The cell medium of organoid models of Langerhans islets, regulating blood glucose levels by insulin secretion, can be analyzed by liquid chromatography-mass spectrometry (LCMS). However, organoid medium complexity is a major challenge, as matrix interferences can reduce sensitivity and selectivity, even with optimized LCMS conditions. By applying preparative agarose gel electrophoresis-electrodialysis (PGE-ED), we were able to decrease the cell medium background signal, allowing for reduced interferences affecting LCMS analysis of human insulin.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
—
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Chromatography A
ISSN
0021-9673
e-ISSN
1873-3778
Volume of the periodical
1717
Issue of the periodical within the volume
FEB
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
7
Pages from-to
464669
UT code for WoS article
001173156700001
EID of the result in the Scopus database
2-s2.0-85185009025