Optimization of saliva sampling methods for analysis of bile acids by UHPLC-MS
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F24%3A00598983" target="_blank" >RIV/68081715:_____/24:00598983 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14110/24:00137239 RIV/65269705:_____/24:00080415
Result on the web
<a href="https://hdl.handle.net/11104/0356553" target="_blank" >https://hdl.handle.net/11104/0356553</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.chroma.2024.465354" target="_blank" >10.1016/j.chroma.2024.465354</a>
Alternative languages
Result language
angličtina
Original language name
Optimization of saliva sampling methods for analysis of bile acids by UHPLC-MS
Original language description
This study investigated methods for sampling bile acids in saliva, a potential non-invasive diagnostic biofluid. Bile acids have been implicated in causing damage and permanent changes to the esophageal mucosa and increasing the risk of developing Barrett's esophagus, a condition that can potentially progress to esophageal cancer. Three saliva collection methods were compared: spitting, Salivette (R) swabs, and Salivette Cortisol (R) swabs. Spitting emerged as the superior method with the highest recoveries and the least interference, likely due to Salivette swabs retaining bile acids or introducing unknown interferences. All saliva samples were analyzed by UHPLC-MS/MS using the Zorbax RRHD Eclipse Plus C18 column (3 x 50 mm, 1.8 mu m) in gradient elution of 0.1 % formic acid in water and methanol. Saliva sample stability was assessed over 14 days, reflecting typical storage times. The levels of detected bile acids were stable for the measured period (RSD <= 22 %) and no degradation was observed. Bile acid levels in saliva fluctuated throughout the day, with the greatest changes observed for glycine-conjugated bile acids after meals. To minimize sampling variability, saliva collection by spitting after overnight fasting is recommended for future studies. Our findings are applicable for standardized bile acid sampling and are currently applied in a large clinical study evaluating bile acids as potential susceptibility markers for Barrett's esophagus diagnostics.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
<a href="/en/project/NU23-08-00303" target="_blank" >NU23-08-00303: Analysis of bile acids as a new, non-invasive method for diagnosing gastroesophageal reflux disease and screening of Barrett’s esophagus.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Chromatography A
ISSN
0021-9673
e-ISSN
1873-3778
Volume of the periodical
1736
Issue of the periodical within the volume
NOV
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
7
Pages from-to
465354
UT code for WoS article
001316072100001
EID of the result in the Scopus database
2-s2.0-85203637473