3D-printed device for time- and cost-efficient sample preparation and DNA fractionation
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F25%3A00642593" target="_blank" >RIV/68081715:_____/25:00642593 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14310/25:00143554 RIV/00216208:11310/25:10509994
Result on the web
<a href="https://hdl.handle.net/11104/0372452" target="_blank" >https://hdl.handle.net/11104/0372452</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.talanta.2024.127461" target="_blank" >10.1016/j.talanta.2024.127461</a>
Alternative languages
Result language
angličtina
Original language name
3D-printed device for time- and cost-efficient sample preparation and DNA fractionation
Original language description
Direct detection of DNA in complex biological samples bears several challenges regarding the selectivity and sensitivity of analyses. Therefore, DNA pre-extraction from bio-fluids is an emerging tool in biologically related fields. Specifically, a newly developing family of liquid biopsy techniques using PCR detection of circulating tumor DNA from blood serum or blood plasma could be significantly improved by harnessing fast and highthroughput DNA sample preparation. To address these needs, a 3D-printed device and a method based on gel electrophoresis combined with electrodialysis for the time-, cost- and labor-efficient preparative separation of DNA fragments from blood was developed. The proposed system also successfully eliminated large DNA fragments from the samples. Recovery for short DNA fragments was reaching up to 80 %. The method was tested onnhuman genomic DNA and blood and blood serum spiked with DNA standards, and it significantly alleviated the signal of matrix DNA.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Talanta
ISSN
0039-9140
e-ISSN
1873-3573
Volume of the periodical
286
Issue of the periodical within the volume
MAY
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
7
Pages from-to
127461
UT code for WoS article
001412356500001
EID of the result in the Scopus database
2-s2.0-85213531037