Spatial structure of chromatin in hybrid cells produced by laser induced fusion studied by optical microscopy.
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081731%3A_____%2F02%3A12030014" target="_blank" >RIV/68081731:_____/02:12030014 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Spatial structure of chromatin in hybrid cells produced by laser induced fusion studied by optical microscopy.
Original language description
In this article we describe a combined system that uses optical tweezers to bring two living cells into contact and optical scalpel to puncuate their membranes at the contact point. This process inititates a fusion of both cells into one hybrid cell containing two nuclei. If the fusion product is viable, these nuclei tend to mix together. The spatial distribution of the nuclear material in the resulting hybrid nucleus is studied by analysis of positions of FISH (fluorescent hybridization in situ) signals of specific genetic loci in automated fluorescence microscope (high resolution cytometer). The obtained results are compared to the signals distribution of FISH in the original cells.
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
BH - Optics, masers and lasers
OECD FORD branch
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Result continuities
Project
<a href="/en/project/IAA1065203" target="_blank" >IAA1065203: The use of combination of laser microbeam and cytometric techniques in the study of the structure and dynamics of human genome</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2002
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
Proceedings of SPIE - The International society for optical engineering. Photonic, devices, and systems II.
ISBN
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ISSN
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e-ISSN
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Number of pages
5
Pages from-to
630-634
Publisher name
SPIE
Place of publication
Prague
Event location
Praha [CZ]
Event date
May 26, 2002
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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