Detecting sequential bond formation using three-dimensional thermal fluctuation analysis

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081731%3A_____%2F09%3A00333759" target="_blank" >RIV/68081731:_____/09:00333759 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Detecting sequential bond formation using three-dimensional thermal fluctuation analysis

Original language description

We present a novel experimental method that solves two key problems in nondestructive mechanical studies of small biomolecules at the single-molecule level, namely the confirmation of single-molecule conditions and the discrimination against nonspecificbinding. A biotin avidin ligand-receptor couple is spanned between a glass slide and a 1 mu m latex particles using short linker molecules. Optical tweezers are used to initiate bond formation and to follow the particles thermal position fluctuations with nanometer spatial and microsecond temporal resolution. Here we show that each step in the specific binding process leads to an abrupt change in the magnitude of the particle's thermal position fluctuations, allowing us to count the number of bonds formed one by one. Moreover, three-dimensional position histograms calculated from the particle's fluctuations can be separated into well-defined categories reflecting different binding conditions.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

BO - Biophysics

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2009

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

ChemPhysChem

ISSN

1439-4235

e-ISSN

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Volume of the periodical

10

Issue of the periodical within the volume

9-10

Country of publishing house

DE - GERMANY

Number of pages

7

Pages from-to

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UT code for WoS article

000267928100028

EID of the result in the Scopus database

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