Identification of individual biofilm-forming bacterial cells using Raman tweezers
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081731%3A_____%2F15%3A00446098" target="_blank" >RIV/68081731:_____/15:00446098 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14110/15:00080989 RIV/00159816:_____/15:00063509
Result on the web
<a href="http://dx.doi.org/10.1117/1.JBO.20.5.051038" target="_blank" >http://dx.doi.org/10.1117/1.JBO.20.5.051038</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1117/1.JBO.20.5.051038" target="_blank" >10.1117/1.JBO.20.5.051038</a>
Alternative languages
Result language
angličtina
Original language name
Identification of individual biofilm-forming bacterial cells using Raman tweezers
Original language description
A method for in vitro identification of individual bacterial cells is presented. The method is based on a combination of optical tweezers for spatial trapping of individual bacterial cells and Raman microspectroscopy for acquisition of spectral "Raman fingerprints" obtained from the trapped cell. Here, Raman spectra were taken from the biofilm-forming cells without the influence of an extracellular matrix and were compared with biofilmnegative cells. Results of principal component analyses of Raman spectra enabled us to distinguish between the two strains of Staphylococcus epidermidis. Thus, we propose that Raman tweezers can become the technique of choice for a clearer understanding of the processes involved in bacterial biofilms which constitute a highly privileged way of life for bacteria, protected from the external environment.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
BH - Optics, masers and lasers
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Biomedical Optics
ISSN
1083-3668
e-ISSN
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Volume of the periodical
20
Issue of the periodical within the volume
5
Country of publishing house
US - UNITED STATES
Number of pages
6
Pages from-to
"051038:1"-"6"
UT code for WoS article
000356241900043
EID of the result in the Scopus database
2-s2.0-84924083195