Polyhydroxyalkanoates in bacterial cells – more than just storage materials

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081731%3A_____%2F16%3A00465097" target="_blank" >RIV/68081731:_____/16:00465097 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.4028/www.scientific.net/MSF.851.20" target="_blank" >http://dx.doi.org/10.4028/www.scientific.net/MSF.851.20</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.4028/www.scientific.net/MSF.851.20" target="_blank" >10.4028/www.scientific.net/MSF.851.20</a>

Result language

angličtina

Original language name

Polyhydroxyalkanoates in bacterial cells – more than just storage materials

Original language description

Since polyhydroxyalkanoates (PHAs) belong among the most widespread storage materials within bacteria genus, it can be assumed that the ability of PHAs accumulation represent significant advantage in the natural environments. Of course, probably the most obvious is the possibility to utilize PHAs when external carbon sources are depleted. Nevertheless, it is likely that PHAs play much more complex role in the stress response of bacteria. Therefore, the aim of this work was to investigate possible influence of PHA accumulation in bacteria on physical properties of the cells and their cytoplasm with respect to possible stress survival. Cells of bacteria Cupriavidus necator H16 with various poly(3-hydroxybutyrate) (PHB) content (25 – 91% of cell dry weight) were subjected to analytical centrifugation employing LUMiSizer. We observed that slope of index instability of bacterial culture increased with rise of PHB content in cells. It indicates that accumulation of PHB granules in bacterial cells affects overall physico-mechanical properties of the cells in particular sedimentation potential and density which might influence their behavior in natural environment such as sedimentation, surface absorption and subsequent biofilm formation. Furthermore, when investigated by Cryo-SEM, the PHB containing cells showed needle-type plastic deformations while these structures were absent in the cells without polymer. This suggests that native intracellular PHB granules reveal completely different mechanical and physico-chemical properties than any other component of bacterial cytoplasm and their flexibility even in deeplyfrozen state is significantly higher than that of PHB isolated from bacterial cells.

Czech name

—

Czech description

—

Type

D - Article in proceedings

CEP classification

JA - Electronics and optoelectronics

OECD FORD branch

—

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Materials Science Forum

ISBN

—

ISSN

1662-9752

e-ISSN

—

Number of pages

6

Pages from-to

20-25

Publisher name

Trans Tech Publications

Place of publication

London

Event location

Brno

Event date

Sep 2, 2016

Type of event by nationality

EUR - Evropská akce

UT code for WoS article

—