Monitoring lipid production in yeast using SEM and Raman spectroscopy

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081731%3A_____%2F17%3A00481587" target="_blank" >RIV/68081731:_____/17:00481587 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Monitoring lipid production in yeast using SEM and Raman spectroscopy

Original language description

The main goal of our project is the development of specialized instrumentation and methodology to monitor metabolic states of microorganisms in order to optimize cultivation process for biotechnological applications. Here the attention is given to the oil-producing yeast strain Metschnikowia pulcherrima. The influence of different cultivation parameters (such as the effects of temperature regime and medium composition) on cells can be monitored. In our investigations on yeast cells scanning electron microscopy (SEM) and Raman spectroscopy techniques were used. Here, SEM uses electron beam to gain information about morphology of cells which reflects cells response on the different cultivation conditions. Consequently, Raman spectroscopy was used for the determination of lipids/oil present in the biomass. Thus, our study targets some factors which could lead to efficient industrial production of oil in selected biotechnological production. The yeast strains (Metschnikowia) were cultivated in medium containing different ratios of C/N (glycerol/yeast extract and/or ammonium sulphate) during a time period of two weeks. On the different days of cultivation, the cells were collected by centrifugation (2000 rpm, 1 min.) and analysed. SEM - the samples of yeast were cultivated as mentioned above and observed by cryo scanning electron microscopy (cryo-SEM).

Czech name

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Czech description

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Type

D - Article in proceedings

CEP classification

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OECD FORD branch

20201 - Electrical and electronic engineering

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

13th Multinational Congress on Microscopy: Book of Abstracts

ISBN

978-953-7941-19-2

ISSN

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e-ISSN

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Number of pages

2

Pages from-to

308-309

Publisher name

Ruder Bošković Institute, Croatian Microscopy Society

Place of publication

Zagreb

Event location

Rovinj

Event date

Sep 24, 2017

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

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