Laser tweezers Raman spectroscopy of E. coli under antibiotic stress in microfluidic chips

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081731%3A_____%2F18%3A00498740" target="_blank" >RIV/68081731:_____/18:00498740 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1117/12.2517933" target="_blank" >http://dx.doi.org/10.1117/12.2517933</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1117/12.2517933" target="_blank" >10.1117/12.2517933</a>

Result language
angličtina
Original language name
Laser tweezers Raman spectroscopy of E. coli under antibiotic stress in microfluidic chips
Original language description
Analyzing the cells in various body fluids can greatly deepen the understanding of the mechanisms governing the cellular physiology. Because of the variability of physiological and metabolic states, it is important to be able to perform such studies on individual cells. Therefore, we developed an optofluidic system in which we precisely manipulated and monitored individual cells of Escherichia coli. We used laser tweezers Raman spectroscopy (LTRS) in a microchamber chip to manipulate and analyze individual E. coli cells. We subjected the cells to antibiotic cefotaxime, and we observed the changes by the time-lapse microscopy and Raman spectroscopy. We found observable changes in the cellularnmorphology (cell elongation) and in Raman spectra, which were consistent with other recently published observations. We tested the capabilities of the optofluidic system and found it to be a reliable and versatile solution for this class of microbiological experiments.
Czech name
—
Czech description
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
21st Czech-Polish-Slovak Optical Conference on Wave and Quantum Aspects of Contemporary Optics. (Proceedings of SPIE 10976)
ISBN
9781510626072
ISSN
0277-786X
e-ISSN
—
Number of pages
8
Pages from-to
—
Publisher name
SPIE
Place of publication
Bellingham
Event location
Lednice
Event date
Sep 3, 2018
Type of event by nationality
EUR - Evropská akce
UT code for WoS article
000455324600043

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