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Analysis of Bacteriophage-Host Interaction by Raman Tweezers

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081731%3A_____%2F20%3A00534715" target="_blank" >RIV/68081731:_____/20:00534715 - isvavai.cz</a>

  • Alternative codes found

    RIV/00216224:14110/20:00114355 RIV/00216305:26310/20:PU137478 RIV/00216208:11310/20:10414371 RIV/00159816:_____/20:00073209

  • Result on the web

    <a href="https://pubs.acs.org/doi/abs/10.1021/acs.analchem.0c01963" target="_blank" >https://pubs.acs.org/doi/abs/10.1021/acs.analchem.0c01963</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1021/acs.analchem.0c01963" target="_blank" >10.1021/acs.analchem.0c01963</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Analysis of Bacteriophage-Host Interaction by Raman Tweezers

  • Original language description

    Bacteriophages, or phages for short, are viruses that replicate in bacteria. The therapeutic and biotechnological potential of phages and their lytic enzymes is of interest for their ability to selectively destroy pathogenic bacteria, including antibiotic-resistant strains. Introduction of phage preparations into medicine, biotechnology, and food industry requires a thorough characterization of phage-host interaction on a molecular level. We employed Raman tweezers to analyze the phage-host interaction of Staphylococcus aureus strain FS159 with a virulent phage JK2 (=812K1/420) of the Myoviridae family and a temperate phage 80 alpha of the Siphoviridae family. We analyzed the timeline of phage-induced molecular changes in infected host cells. We reliably detected the presence of replicating phages in bacterial cells within 5 min after infection. Our results lay the foundations for building a Raman-based diagnostic instrument capable of real-time, in vivo, in situ, nondestructive characterization of the phage-host relationship on the level of individual cells, which has the potential of importantly contributing to the development of phage therapy and enzybiotics.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10306 - Optics (including laser optics and quantum optics)

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2020

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Analytical Chemistry

  • ISSN

    0003-2700

  • e-ISSN

  • Volume of the periodical

    92

  • Issue of the periodical within the volume

    18

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    8

  • Pages from-to

    12304-12311

  • UT code for WoS article

    000572832900029

  • EID of the result in the Scopus database

    2-s2.0-85094979024