Analysis of Bacteriophage-Host Interaction by Raman Tweezers

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081731%3A_____%2F20%3A00534715" target="_blank" >RIV/68081731:_____/20:00534715 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14110/20:00114355 RIV/00216305:26310/20:PU137478 RIV/00216208:11310/20:10414371 RIV/00159816:_____/20:00073209
Result on the web
<a href="https://pubs.acs.org/doi/abs/10.1021/acs.analchem.0c01963" target="_blank" >https://pubs.acs.org/doi/abs/10.1021/acs.analchem.0c01963</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/acs.analchem.0c01963" target="_blank" >10.1021/acs.analchem.0c01963</a>

Result language
angličtina
Original language name
Analysis of Bacteriophage-Host Interaction by Raman Tweezers
Original language description
Bacteriophages, or phages for short, are viruses that replicate in bacteria. The therapeutic and biotechnological potential of phages and their lytic enzymes is of interest for their ability to selectively destroy pathogenic bacteria, including antibiotic-resistant strains. Introduction of phage preparations into medicine, biotechnology, and food industry requires a thorough characterization of phage-host interaction on a molecular level. We employed Raman tweezers to analyze the phage-host interaction of Staphylococcus aureus strain FS159 with a virulent phage JK2 (=812K1/420) of the Myoviridae family and a temperate phage 80 alpha of the Siphoviridae family. We analyzed the timeline of phage-induced molecular changes in infected host cells. We reliably detected the presence of replicating phages in bacterial cells within 5 min after infection. Our results lay the foundations for building a Raman-based diagnostic instrument capable of real-time, in vivo, in situ, nondestructive characterization of the phage-host relationship on the level of individual cells, which has the potential of importantly contributing to the development of phage therapy and enzybiotics.
Czech name
—
Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10306 - Optics (including laser optics and quantum optics)

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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