Gold nanosystems for the detection of molecules using surface-enhanced Raman scatterings (SERS)

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081731%3A_____%2F22%3A00567652" target="_blank" >RIV/68081731:_____/22:00567652 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Gold nanosystems for the detection of molecules using surface-enhanced Raman scatterings (SERS)

Original language description

Raman spectroscopy is a non-destructive analytical technique to analyze the chemical structure of molecules by a phenomenon known as Raman scattering, which occurs by an inelastic interaction of photons with the valence electrons in molecular bonds. However, Raman scattering can be hard to observe due to other, more frequent phenomena, such as Rayleigh scattering or fluorescence. SERS (surface-enhanced Raman spectroscopy) uses localized surface plasmon resonance (LSPR) of metal nanostructures to amplify Raman scattering. LSPR is a coherent oscillation of conduction electrons that arises from the interaction of electromagnetic radiation with metal nanostructures. The amplification of Raman scattering occurs when the analyte is adsorbed on the surface of such nanostructure and the strong localized electric field interacts with the electrons in its molecular bonds. Signal amplification of several orders of magnitude can be achieved, commonly 103 or more. In our work, we determined the presence of a selected bacterial species by multi-functionalized golden nanoparticles called SERS-tags, which have their surface modified with an antibody and a Raman reporter. The antibody allows the nanoparticles to bind to the surface of a concrete bacterial species based on the antigen-antibody affinity. When the targeted bacterium is covered with the nanoparticles, the Raman reporter signal is amplified by SERS, providing specific and strong Raman response. Therefore, when the Raman reporter signal is detected in a sample, it confirms the presence of the specific bacterium on a single-cell level.

Czech name

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Czech description

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Type

D - Article in proceedings

CEP classification

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OECD FORD branch

10406 - Analytical chemistry

Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2022

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

LA62. Sborník příspěvků multioborové konference LASER62

ISBN

978-80-87441-30-5

ISSN

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e-ISSN

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Number of pages

3

Pages from-to

9-11

Publisher name

Ústav přístrojové techniky AV ČR

Place of publication

Brno

Event location

Lednice

Event date

Nov 9, 2022

Type of event by nationality

EUR - Evropská akce

UT code for WoS article

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