Impact of secondary ice in a frozen NaCl freeze-concentrated solution on the extent of methylene blue aggregation
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081731%3A_____%2F24%3A00581837" target="_blank" >RIV/68081731:_____/24:00581837 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14310/24:00135481
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S0378517323011134" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0378517323011134</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.ijpharm.2023.123691" target="_blank" >10.1016/j.ijpharm.2023.123691</a>
Alternative languages
Result language
angličtina
Original language name
Impact of secondary ice in a frozen NaCl freeze-concentrated solution on the extent of methylene blue aggregation
Original language description
Freezing and lyophilization have been utilized for decades to stabilize pharmaceutical and food products. Freezing a solution that contains dissolved salt and/or organic matter produces pure primary ice crystal grains separated by freeze-concentrated solutions (FCS). The microscopic size of the primary ice crystals depends on the cooling conditions and the concentration of the solutes. It is generally accepted that primary ice crystals size influences the rate of sublimation and also can impact physico-chemical behaviour of the species in the FCS. This article, however, presents a case where the secondary ice formed inside the FCS plays a critical role. We microscoped the structures of ice-cast FCS with an environmental scanning electron microscope and applied the aggregation-sensitive spectroscopic probe methylene blue to determine how the microstructure affects the mo-lecular arrangement. We show that slow cooling at-50 degrees C produces large salt crystals with a small specific surface, resulting in a high degree of molecular aggregation within the FCS. In contrast, fast liquid nitrogen cooling yields an ultrafine structure of salt crystals having a large specific surface area and, therefore, inducing smaller aggregation. The study highlights a critical role of secondary ice in solute aggregation and introduces methylene blue as a molecular probe to investigate freezing behaviour of aqueous systems with crystalline solute.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
20201 - Electrical and electronic engineering
Result continuities
Project
<a href="/en/project/GA22-25799S" target="_blank" >GA22-25799S: Correlative Fluorescence Microscopy and Advanced Low-energy ESEM for Imaging of Immuno-Labelled Wet Biological Samples.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
International Journal of Pharmaceutics
ISSN
0378-5173
e-ISSN
1873-3476
Volume of the periodical
650
Issue of the periodical within the volume
25 January
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
10
Pages from-to
123691
UT code for WoS article
001138921200001
EID of the result in the Scopus database
2-s2.0-85180113202