“There’s plenty of room at the bottom”: deep brain imaging with holographic endo-microscopy

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081731%3A_____%2F24%3A00588514" target="_blank" >RIV/68081731:_____/24:00588514 - isvavai.cz</a>

Result on the web

<a href="https://www.spiedigitallibrary.org/journals/neurophotonics/volume-11/issue-S1/S11504/Theres-plenty-of-room-at-the-bottom--deep-brain/10.1117/1.NPh.11.S1.S11504.full#_=_" target="_blank" >https://www.spiedigitallibrary.org/journals/neurophotonics/volume-11/issue-S1/S11504/Theres-plenty-of-room-at-the-bottom--deep-brain/10.1117/1.NPh.11.S1.S11504.full#_=_</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1117/1.NPh.11.S1.S11504" target="_blank" >10.1117/1.NPh.11.S1.S11504</a>

Result language

angličtina

Original language name

“There’s plenty of room at the bottom”: deep brain imaging with holographic endo-microscopy

Original language description

Significance: Over more than 300 years, microscopic imaging keeps providing fundamental insights into the mechanisms of living organisms. Seeing microscopic structures beyond the reach of free-space light-based microscopy, however, requires dissection of the tissue—an intervention seriously disturbing its physiological functions. The hunt for low-invasiveness tools has led a growing community of physicists and engineers into the realm of complex media photonics. One of its activities represents exploiting multimode optical fibers (MMFs) as ultra-thin endoscopic probes. Employing wavefront shaping, these tools only recently facilitated the first peeks at cells and their sub-cellular compartments at the bottom of the mouse brain with the impact of micro-scale tissue damage. Aim: Here, we aim to highlight advances in MMF-based holographic endo-microscopy facilitating microscopic imaging throughout the whole depth of the mouse brain. Approach: We summarize the important technical and methodological prerequisites for stabile high-resolution imaging in vivo. Results: We showcase images of the microscopic building blocks of brain tissue, including neurons, neuronal processes, vessels, intracellular calcium signaling, and red blood cell velocity in individual vessels. Conclusions: This perspective article helps to understand the complexity behind the technology of holographic endo-microscopy, summarizes its recent advances and challenges, and stimulates the mind of the reader for further exploitation of this tool in the neuroscience research. (c) The Authors. Published by SPIE under a Creative Commons Attribution 4.0 International License.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10306 - Optics (including laser optics and quantum optics)

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Neurophotonics

ISSN

2329-423X

e-ISSN

2329-4248

Volume of the periodical

11

Issue of the periodical within the volume

S1

Country of publishing house

US - UNITED STATES

Number of pages

11

Pages from-to

S11504

UT code for WoS article

001316474200005

EID of the result in the Scopus database

2-s2.0-85204424233