Disruption of Extracellular Matrix and Perineuronal Nets Modulates Extracellular Space Volume and Geometry
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081731%3A_____%2F25%3A00617960" target="_blank" >RIV/68081731:_____/25:00617960 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11130/25:10489502
Result on the web
<a href="https://www.jneurosci.org/content/45/8/e0517242024" target="_blank" >https://www.jneurosci.org/content/45/8/e0517242024</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1523/JNEUROSCI.0517-24.2024" target="_blank" >10.1523/JNEUROSCI.0517-24.2024</a>
Alternative languages
Result language
angličtina
Original language name
Disruption of Extracellular Matrix and Perineuronal Nets Modulates Extracellular Space Volume and Geometry
Original language description
Extracellular matrix (ECM) is a network of macromolecules which has two forms-perineuronal nets (PNNs) and a diffuse ECM (dECM)-both influence brain development, synapse formation, neuroplasticity, CNS injury and progression of neurodegenerative diseases. ECM remodeling can influence extrasynaptic transmission, mediated by diffusion of neuroactive substances in the extracellular space (ECS). In this study we analyzed how disrupted PNNs and dECM influence brain diffusibility. Two months after oral treatment of rats with 4-methylumbelliferone (4-MU), an inhibitor of hyaluronan (HA) synthesis, we found downregulated staining for PNNs, HA, chondroitin sulfate proteoglycans, and glial fibrillary acidic protein. These changes were enhanced after 4 and 6 months and were reversible after a normal diet. Morphometric analysis further indicated atrophy of astrocytes. Using real-time iontophoretic method dysregulation of ECM resulted in increased ECS volume fraction alpha in the somatosensory cortex by 35%, from alpha = 0.20 in control rats to alpha = 0.27 after the 4-MU diet. Diffusion-weighted magnetic resonance imaging revealed a decrease of mean diffusivity and fractional anisotropy (FA) in the cortex, hippocampus, thalamus, pallidum, and spinal cord. This study shows the increase in ECS volume, a loss of FA, and changes in astrocytes due to modulation of PNNs and dECM that could affect extrasynaptic transmission, cell-to-cell communication, and neural plasticity.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
20601 - Medical engineering
Result continuities
Project
<a href="/en/project/LM2023050" target="_blank" >LM2023050: National Infrastructure for Biological and Medical Imaging</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Neuroscience
ISSN
0270-6474
e-ISSN
1529-2401
Volume of the periodical
45
Issue of the periodical within the volume
8
Country of publishing house
US - UNITED STATES
Number of pages
14
Pages from-to
e0517242024
UT code for WoS article
001433399000007
EID of the result in the Scopus database
2-s2.0-85219548553