Assembly for semi-correlation analysis of samples using cryogenic scanning electron microscopy and cryogenic Raman micro-spectroscopy

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081731%3A_____%2F25%3A00635880" target="_blank" >RIV/68081731:_____/25:00635880 - isvavai.cz</a>

Alternative codes found

RIV/00216305:26310/26:0197994

Result on the web

<a href="https://iopscience.iop.org/article/10.1088/1361-6501/adc94a" target="_blank" >https://iopscience.iop.org/article/10.1088/1361-6501/adc94a</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1088/1361-6501/adc94a" target="_blank" >10.1088/1361-6501/adc94a</a>

Result language

angličtina

Original language name

Assembly for semi-correlation analysis of samples using cryogenic scanning electron microscopy and cryogenic Raman micro-spectroscopy

Original language description

Correlative imaging, integrating diverse observational methods, has become increasingly vital for comprehensive sample analysis by linking distinct datasets that are accessible only through individual techniques. This study combines cryogenic scanning electron microscopy (cryo-SEM) and cryogenic Raman micro-spectroscopy (cryo-Raman), which together offer extensive insight into sample analysis. Cryo-SEM effectively investigates the surface, structure, and morphology, while cryo-Raman excels in detecting and identifying chemical composition. To integrate these techniques, we developed a novel assembly compatible with commercial cryo-SEM sample holders. The assembly is designed for precise sample positioning and observation at cryogenic temperatures of liquid nitrogen. This setup, controlled by LabVIEW software, includes a Dewar vessel containing liquid nitrogen, a 3D-printed stage for mounting sample holders, and a piezoelectric stage for XY motion, with z axis adjustments connected to the Raman spectrometer table. A plexiglass cover minimizes contamination and preserves sample integrity during analysis. The assembly was validated by first analyzing polystyrene at cryogenic temperatures to ensure its functionality. Subsequent tests on Cupriavidus necator H16-bacteria capable of producing polyhydroxyalkanoates (PHAs), a type of biodegradable polyester-demonstrated the system's efficiency. PHAs, which are of interest as a sustainable alternative to petrochemical plastics, can be produced from industrial waste streams. Our results show that the combination of cryo-SEM and cryo-Raman is suitable for studying these microorganisms and permits a deeper understanding of the properties of polymer granules in microbial cells. This integrated approach represents a significant advance in semi-correlative imaging, providing more efficient and detailed analyses for future research in microbial physiology production and related fields.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

20201 - Electrical and electronic engineering

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Measurement Science and Technology

ISSN

0957-0233

e-ISSN

1361-6501

Volume of the periodical

36

Issue of the periodical within the volume

5

Country of publishing house

GB - UNITED KINGDOM

Number of pages

10

Pages from-to

055903

UT code for WoS article

001473344200001

EID of the result in the Scopus database

2-s2.0-105003384007