Self-entrapment of Azotobacter vinelandii cultures by gelation of their exopolysaccharides: A way towards next-generation bioinoculants

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081731%3A_____%2F25%3A00643219" target="_blank" >RIV/68081731:_____/25:00643219 - isvavai.cz</a>

Result on the web

<a href="https://www.sciencedirect.com/science/article/pii/S0144861725003881" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0144861725003881</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.carbpol.2025.123607" target="_blank" >10.1016/j.carbpol.2025.123607</a>

Result language

angličtina

Original language name

Self-entrapment of Azotobacter vinelandii cultures by gelation of their exopolysaccharides: A way towards next-generation bioinoculants

Original language description

Encapsulation of Plant Growth-Promoting Rhizobacteria (PGPR) in hydrogel carriers is a cutting-edge approach in developing agricultural bioinoculants, aiming to improve soil fertility and crop yield. Hydrogels provide protection against environmental stress, though traditional methods using external gel-forming agents limit economic viability. This study presents a novel approach, demonstrating the entrapment of Azotobacter vinelandii, a promising PGPR, within a gel matrix formed by Ca2+-induced crosslinking of its own exopolysaccharide, alginate. Among the five strains evaluated, A. vinelandii DSM 87, DSM 720, and DSM 13529 showed the highest alginate production, peaking at 4.9 +/- 0.6 g/L, 3.5 +/- 0.5 g/L, 3.8 +/- 0.8 g/L, and enabling stable gel formation by the fourth day of cultivation. These strains also exhibited molecular weights and chemical structures of alginate suitable for effective gelation upon Ca2+addition. Additionally, these strains demonstrated significant plant growth-promoting activities, including indole acetic acid production (up to 10.5 mu g/mL), siderophore release, and phosphate solubilization, further validating their potential for sustainable bioinoculant production. Finally, the viability of the A.vinelandii cells released from the gels was experimentally verified. Our findings support a feasible, cost-effective method for bioinoculant production that leverages A. vinelandii's intrinsic capabilities, offering a sustainable alternative to conventional agricultural practices.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10606 - Microbiology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Carbohydrate Polymers

ISSN

0144-8617

e-ISSN

1879-1344

Volume of the periodical

360

Issue of the periodical within the volume

15 July

Country of publishing house

GB - UNITED KINGDOM

Number of pages

11

Pages from-to

123607

UT code for WoS article

001474799900001

EID of the result in the Scopus database

2-s2.0-105002798618