Fast sex identification in wild mammals using PCR amplification of the Sry gene.

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081766%3A_____%2F03%3A67030051" target="_blank" >RIV/68081766:_____/03:67030051 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Fast sex identification in wild mammals using PCR amplification of the Sry gene.
Original language description
Several primer sets were described for PCR amplification of the Sry gene that became very suitable method for sex identification in mammals. We tested applicability of two primer sets for sex identification in one bat and 12 rodent species from Central Europe. Rapid DNA extraction with non-ionic detergents, which is very suitable for large-scale population studies, was used. Primers SRY-A from Pomp et al. (1995) did not amplify Sry fragment in males of Myotis myotis, four species of Microtus, and Clethrionomys glareolus, while amplification was successful in four species of Apodemus, two species of Mus, and Sciurus vulgaris. On the other hand, primers from Sanchez et al. (1996) gave clear Sry band in males of all tested species, only with one exception, i.e. male of Sciurus vulgaris.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EG - Zoology
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2003
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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