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Ribotyping and whole-cell protein analysis of spirochetes isolated from arthropods in the Czech Republic

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081766%3A_____%2F08%3A00315032" target="_blank" >RIV/68081766:_____/08:00315032 - isvavai.cz</a>

  • Alternative codes found

    RIV/00216224:14310/08:00024347

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Ribotyping and whole-cell protein analysis of spirochetes isolated from arthropods in the Czech Republic

  • Original language description

    Six Borrelia burgdorferi sensu lato isolates were recovered from Ixodes ricinus ticks and one strain of spirochete with uncertain taxonomic position isolated from Culex pipiens mosquito collected in the Valtice area (South Moravia, Czech Republic), as well as representative type strains, were further characterized by ribotyping and whole-cell protein fingerprinting. The ribotype patterns obtained with HindIII and EcoRV showed good correlations with the recently proposed genotypes and grouped each genospecies in a well-separated cluster. The whole-cell protein profiles of genospecies were more heterogenous than the ribotype patterns. The non-identified spirochetal strain was clearly separated from the Borrelia isolates in both methods thus prooving their good capacity to characterize Borrelia burgdorferi sensu lato strains.

  • Czech name

    Ribotypizace a analýza celobuněčných proteinů spirochét izolovaných z členovců v České republice

  • Czech description

    Šest izolátů Borrelia burgdorferi sensu lato získaných z klíštěte Ixodes ricinus a jeden kmen spirochéty dosud taxonomicky nezařazené z komára Culex pipiens na lokalitě Valtice (Jižní Morava, Česká republika) se podrobily ribotypizaci a analýze celobuněčných proteinů. Ribotypizační profily získané po štěpení enzymy HindIII a EcoRV vykazovaly shodu s nově navrženými genotypy a tvořily dobře odlišitelné skupiny. Analýza celobuněčných proteinů poskytovala variabilnější profily. Dosud nezařazený kmen komáříspirochéty tvořil separátní skupinu a odlišoval se od borrelií, což ukazuje, že obě metody se jeví vhodnými pro charakteristiku spirochétálních kmenů.

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    EE - Microbiology, virology

  • OECD FORD branch

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2008

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Annals of Agricultural and Environmental Medicine

  • ISSN

    1232-1966

  • e-ISSN

  • Volume of the periodical

    15

  • Issue of the periodical within the volume

    2

  • Country of publishing house

    PL - POLAND

  • Number of pages

    6

  • Pages from-to

  • UT code for WoS article

    000261767500009

  • EID of the result in the Scopus database