Molecular differentiation of three loach species (Pisces, Cobitidae) based on the nuclear 5S rDNA marker
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081766%3A_____%2F11%3A00384520" target="_blank" >RIV/68081766:_____/11:00384520 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.3409/fb59_3-4.141-145" target="_blank" >http://dx.doi.org/10.3409/fb59_3-4.141-145</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3409/fb59_3-4.141-145" target="_blank" >10.3409/fb59_3-4.141-145</a>
Alternative languages
Result language
angličtina
Original language name
Molecular differentiation of three loach species (Pisces, Cobitidae) based on the nuclear 5S rDNA marker
Original language description
The diversity of the 5S rDNA fragment among three loach species: Cobitis taenia, C. elongatoides and Sabanejewia aurata was investigated using universal PCR primers for this gene. Three amplification products were obtained: 220 bp length for C. taenia and C. elongatoides, and 330 bp for S. aurata. Two amplicons with the same length (in Cobitis) were digested with TaqI restriction endonuclease. This enzyme found one restriction site T/CGA in the C. elongatoides fragment, while in the case of C. taenia nocleavage effect was observed. On this basis we constructed an easy and cheap method for loach species discrimination. It seems adequate for effective support of conservation initiatives for endangered loathes.
Czech name
—
Czech description
—
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
—
Result continuities
Project
—
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Folia Biologica-Krakow
ISSN
0015-5497
e-ISSN
—
Volume of the periodical
59
Issue of the periodical within the volume
3
Country of publishing house
PL - POLAND
Number of pages
5
Pages from-to
141-145
UT code for WoS article
000293365100009
EID of the result in the Scopus database
—