Lessons learned from microsatellite development for nonmodel organisms using 454 pyrosequencing

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081766%3A_____%2F13%3A00390981" target="_blank" >RIV/68081766:_____/13:00390981 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1111/jeb.12077" target="_blank" >http://dx.doi.org/10.1111/jeb.12077</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/jeb.12077" target="_blank" >10.1111/jeb.12077</a>

Result language

angličtina

Original language name

Lessons learned from microsatellite development for nonmodel organisms using 454 pyrosequencing

Original language description

Microsatellites, also known as simple sequence repeats (SSRs), are among the most commonly used marker types in evolutionary and ecological studies. Next Generation Sequencing techniques such as 454 pyrosequencing allow the rapid development of microsatellite markers in nonmodel organisms. 454 pyrosequencing is a straightforward approach to develop a high number of microsatellite markers. Therefore, developing microsatellites using 454 pyrosequencing has become the method of choice for marker development. Here, we describe a user friendly way of microsatellite development from 454 pyrosequencing data and analyse data sets of 17 nonmodel species (plants, fungi, invertebrates, birds and a mammal) for microsatellite repeats and flanking regions suitable for primer development. We then compare the numbers of successfully lab-tested microsatellite markers for the various species and furthermore describe diverse challenges that might arise in different study species, for example, large genom

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Evolutionary Biology

ISSN

1010-061X

e-ISSN

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Volume of the periodical

26

Issue of the periodical within the volume

3

Country of publishing house

GB - UNITED KINGDOM

Number of pages

12

Pages from-to

600-611

UT code for WoS article

000314988900013

EID of the result in the Scopus database

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