Experimental validation of small mammal gut microbiota sampling from faeces and from the caecum after death

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081766%3A_____%2F21%3A00543154" target="_blank" >RIV/68081766:_____/21:00543154 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11310/21:10434282

Result on the web

<a href="https://www.nature.com/articles/s41437-021-00445-6" target="_blank" >https://www.nature.com/articles/s41437-021-00445-6</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41437-021-00445-6" target="_blank" >10.1038/s41437-021-00445-6</a>

Result language

angličtina

Original language name

Experimental validation of small mammal gut microbiota sampling from faeces and from the caecum after death

Original language description

Data on the gut microbiota (GM) of wild animals are key to studies on evolutionary biology (host–GM interactions under natural selection), ecology and conservation biology (GM as a fitness component closely connected to the environment). Wildlife GM sampling often requires non-invasive techniques or sampling from dead animals. In a controlled experiment profiling microbial 16S rRNA in 52 house mice (Mus musculus) from eight families and four genetic backgrounds, we studied the effects of live- and snap-trapping on small mammal GM and evaluated the suitability of microbiota from non-fresh faeces as a proxy for caecal GM. We compared CM from individuals sampled 16–18 h after death with those in live traps and caged controls, and caecal and faecal GM collected from mice in live-traps. Sampling delay did not affect GM composition, validating data from fresh cadavers or snap-trapped animals. Animals trapped overnight displayed a slight but significant difference in GM composition to the caged controls, though the change only had negligible effect on GM diversity, composition and inter-individual divergence. Hence, the trapping process appears not to bias GM profiling. Despite their significant difference, caecal and faecal microbiota were correlated in composition and, to a lesser extent, diversity. Both showed congruent patterns of inter-individual divergence following the natural structure of the dataset. Thus, the faecal microbiome represents a good non-invasive proxy of the caecal microbiome, making it suitable for detecting biologically relevant patterns. However, care should be taken when analysing mixed datasets containing both faecal and caecal samples.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10613 - Zoology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Heredity

ISSN

0018-067X

e-ISSN

1365-2540

Volume of the periodical

127

Issue of the periodical within the volume

2

Country of publishing house

GB - UNITED KINGDOM

Number of pages

10

Pages from-to

141-150

UT code for WoS article

000655744800001

EID of the result in the Scopus database

2-s2.0-85106677213