In situ reverse transcription: the magic of strength and anonymity

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F10%3A00345708" target="_blank" >RIV/68378041:_____/10:00345708 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
In situ reverse transcription: the magic of strength and anonymity
Original language description
In this study, we describe an approach that enables a highly specific, effective and fast detection of polyadenylated RNA sequences in situ at the light and electron microscopy levels. The method developed is based on the incorporation of 5-bromo-2?-deoxyuridine into the growing cDNAstrand by means of the reverse transcriptase. We have shown that unlike the previously used deoxyuridine tagged with biotin or digoxigenin,5-bromo-2-deoxyuridine is invisible? in the DNA?DNA duplex but easily detectable in theDNA?RNA duplex. We have also shown that the replacement of deoxythymidine by 5-bromo-2-deoxyuridine considerably stabilizes the growing DNA?RNA duplex, thus enabling the one-step detection of polyadenylated RNA in structurally well-preserved cells.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EA - Morphology and cytology
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2010
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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