Heterogeneity of Astrocytes: From Development to Injury - Single Cell Gene Expression
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F13%3A00398761" target="_blank" >RIV/68378041:_____/13:00398761 - isvavai.cz</a>
Alternative codes found
RIV/86652036:_____/13:00398761
Result on the web
<a href="http://dx.doi.org/10.1371/journal.pone.0069734" target="_blank" >http://dx.doi.org/10.1371/journal.pone.0069734</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1371/journal.pone.0069734" target="_blank" >10.1371/journal.pone.0069734</a>
Alternative languages
Result language
angličtina
Original language name
Heterogeneity of Astrocytes: From Development to Injury - Single Cell Gene Expression
Original language description
Astrocytes perform control and regulatory functions in the central nervous system; heterogeneity among them is still a matter of debate due to limited knowledge of their gene expression profiles and functional diversity. To unravel astrocyte heterogeneity during postnatal development and after focal cerebral ischemia, we employed single-cell gene expression profiling in acutely isolated cortical GFAP/EGFP-positive cells. Using a microfluidic qPCR platform, we profiled 47 genes encoding glial markers andion channels/transporters/receptors participating in maintaining K+ and glutamate homeostasis per cell. Self-organizing maps and principal component analyses revealed three subpopulations within 10-50 days of postnatal development (P10-P50). The first subpopulation, mainly immature glia from P10, was characterized by high transcriptional activity of all studied genes, including polydendrocytic markers. The second subpopulation (mostly from P20) was characterized by low gene transcript l
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FH - Neurology, neuro-surgery, nuero-sciences
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GA13-02154S" target="_blank" >GA13-02154S: Gene expression profiling and functional characterization of glial cell subpopulations following ischemic brain injury</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
PLoS ONE
ISSN
1932-6203
e-ISSN
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Volume of the periodical
8
Issue of the periodical within the volume
8
Country of publishing house
US - UNITED STATES
Number of pages
15
Pages from-to
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UT code for WoS article
000324465000022
EID of the result in the Scopus database
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