Specific degradation of phosphatidylglycerol is necessary for proper mitochondrial morphology and function
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F16%3A00451032" target="_blank" >RIV/68378041:_____/16:00451032 - isvavai.cz</a>
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S000527281500211X?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S000527281500211X?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.bbabio.2015.10.004" target="_blank" >10.1016/j.bbabio.2015.10.004</a>
Alternative languages
Result language
angličtina
Original language name
Specific degradation of phosphatidylglycerol is necessary for proper mitochondrial morphology and function
Original language description
In yeast, phosphatidylglycerol (PG) is a minor phospholipid under standard conditions it can be utilized for cardiolipin (CL) biosynthesis by CL synthase, Crd1p, or alternatively degraded by the phospholipase Pgc1p. The Saccharomyces cerevisiae deletion mutants crd1 Delta and pgc1 Delta both accumulate PG. Based on analyses of the phospholipid content of pgc1 Delta, and crd1 Delta yeast, we revealed that in yeast mitochondria, two separate pools of PG are present, which differ in their fatty acid composition and accessibility for Pgcl p-catalyzed degradation. In contrast to CL-deficient crd1 Delta yeast, the pgc1 Delta mutant contains normal levels of CL. This makes the pgc1 Delta strain a suitable model to study the effect of accumulation of PG per se. Using fluorescence microscopy, we show that accumulation of PG with normal levels of CL resulted in increased fragmentation of mitochondria, while in the absence of CL, accumulation of PG led to the formation of large mitochondrial sheets. We also show that pgc1 Delta mitochondria exhibited increased respiration rates due to increased activity of cytochrome c oxidase. Taken together, our results indicate that not only a lack of anionic phospholipids, but also excess PG, or unbalanced ratios of anionic phospholipids in mitochondrial membranes, have harmful consequences on mitochondrial morphology and function. (C) 2015 Elsevier B.V. All rights reserved.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10602 - Biology (theoretical, mathematical, thermal, cryobiology, biological rhythm), Evolutionary biology
Result continuities
Project
<a href="/en/project/GA15-10641S" target="_blank" >GA15-10641S: Specific plasma membrane microdomains in regulation of aging</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Biochimica Et Biophysica Acta-Bioenergetics
ISSN
0005-2728
e-ISSN
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Volume of the periodical
1857
Issue of the periodical within the volume
1
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
11
Pages from-to
34-45
UT code for WoS article
000366771700005
EID of the result in the Scopus database
2-s2.0-84945917809