Injectable Extracellular Matrix Hydrogels as Scaffolds for Spinal Cord Injury Repair
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F16%3A00458297" target="_blank" >RIV/68378041:_____/16:00458297 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11130/16:10323724
Result on the web
<a href="http://dx.doi.org/10.1089/ten.tea.2015.0422" target="_blank" >http://dx.doi.org/10.1089/ten.tea.2015.0422</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1089/ten.tea.2015.0422" target="_blank" >10.1089/ten.tea.2015.0422</a>
Alternative languages
Result language
angličtina
Original language name
Injectable Extracellular Matrix Hydrogels as Scaffolds for Spinal Cord Injury Repair
Original language description
Restoration of lost neuronal function after spinal cord injury (SCI) still remains a big challenge for current medicine. One important repair strategy is bridging the SCI lesion with a supportive and stimulatory milieu that would enable axonal rewiring. Injectable extracellular matrix (ECM)-derived hydrogels have been recently reported to have neurotrophic potential in vitro. In this study, we evaluated the presumed neuroregenerative properties of ECM hydrogels in vivo in the acute model of SCI. ECM hydrogels were prepared by decellularization of porcine spinal cord (SC) or porcine urinary bladder (UB), and injected into a spinal cord hemisection cavity. Histological analysis and real-time qPCR were performed at 2, 4, and 8 weeks postinjection. Both types of hydrogels integrated into the lesion and stimulated neovascularization and axonal ingrowth into the lesion. On the other hand, massive infiltration of macrophages into the lesion and rapid hydrogel degradation did not prevent cyst formation, which progressively developed over 8 weeks. No significant differences were found between SC-ECM and UB-ECM. Gene expression analysis revealed significant downregulation of genes related to immune response and inflammation in both hydrogel types at 2 weeks post SCI. A combination of human mesenchymal stem cells with SC-ECM did not further promote ingrowth of axons and blood vessels into the lesion, when compared with the SC-ECM hydrogel alone. In conclusion, both ECM hydrogels bridged the lesion cavity, modulated the innate immune response, and provided the benefit of a stimulatory substrate for in vivo neural tissue regeneration. However, fast hydrogel degradation might be a limiting factor for the use of native ECM hydrogels in the treatment of acute SCI.
Czech name
—
Czech description
—
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FH - Neurology, neuro-surgery, nuero-sciences
OECD FORD branch
—
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Tissue Engineering
ISSN
1937-3341
e-ISSN
—
Volume of the periodical
22
Issue of the periodical within the volume
3-4
Country of publishing house
US - UNITED STATES
Number of pages
12
Pages from-to
306-317
UT code for WoS article
000369987900013
EID of the result in the Scopus database
2-s2.0-84959062446