The effect of medium composition on deposition of collagen type 1 and expression of osteogenic genes in mesenchymal stem cells derived from human adipose tissue and bone marrow

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F17%3A00469068" target="_blank" >RIV/68378041:_____/17:00469068 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11130/17:10373770

Result on the web

<a href="http://dx.doi.org/10.1016/j.procbio.2016.10.011" target="_blank" >http://dx.doi.org/10.1016/j.procbio.2016.10.011</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.procbio.2016.10.011" target="_blank" >10.1016/j.procbio.2016.10.011</a>

Result language

angličtina

Original language name

The effect of medium composition on deposition of collagen type 1 and expression of osteogenic genes in mesenchymal stem cells derived from human adipose tissue and bone marrow

Original language description

The two mesenchymal stem cell (MSC) populations that have gained most attention in relation to bone tissue engineering are adipose tissue (AT) MSCs and bone marrow (BM) MSCs. The purpose of this study was to investigate the ability of human BM-MSCs and AT-MSCs to survive, proliferate and deposit collagen type 1 when cultured on polycaprolactone nanofiber scaffolds and to ascertain the effect of medium composition on collagen type 1 formation and expression of osteogenic genes. The cells were seeded on polycaprolactone nanofiber scaffolds and cultured in three different types of media that differed by the presence of ascorbic acid,beta-glycerophosphate and dexamethasone, that are typical components used for osteogenic differentiation of MSCs in vitro. nnIn summary, AT-MSCs were proliferating significantly faster than BM-MSCs. AT-MSCs also showed better ability to deposit collagen type 1 and had a higher expression of early osteogenic markers, whereas BM-MSCs had higher expression of late osteogenic markers. This suggests that MSCs from diverse sources have different attributes and with respect to osteogenic differentiation, AT-MSCs are more immature compared to BM-MSCs. Collagen formation was depending on medium composition and the organization of collagen type I appeared to be influenced by the presence of dexamethasone.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10601 - Cell biology

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Process Biochemistry

ISSN

1359-5113

e-ISSN

—

Volume of the periodical

59

Issue of the periodical within the volume

B

Country of publishing house

GB - UNITED KINGDOM

Number of pages

8

Pages from-to

321-328

UT code for WoS article

000412376700023

EID of the result in the Scopus database

2-s2.0-85001969692