DNA Damage Potential of Engine Emissions Measured In Vitro by Micronucleus Test in Human Bronchial Epithelial Cells
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F17%3A00469664" target="_blank" >RIV/68378041:_____/17:00469664 - isvavai.cz</a>
Alternative codes found
RIV/68407700:21220/17:00303481 RIV/00216208:11310/17:10369600 RIV/00027162:_____/17:N0000047
Result on the web
<a href="http://dx.doi.org/10.1111/bcpt.12693" target="_blank" >http://dx.doi.org/10.1111/bcpt.12693</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/bcpt.12693" target="_blank" >10.1111/bcpt.12693</a>
Alternative languages
Result language
angličtina
Original language name
DNA Damage Potential of Engine Emissions Measured In Vitro by Micronucleus Test in Human Bronchial Epithelial Cells
Original language description
Internal combustion engine emissions belong among the major anthropogenic sources of air pollution in urban areas. According to the International Agency for Research on Cancer, there is sufficient evidence of the carcinogenicity of diesel exhaust in human beings. Although alternative fuels, mainly biodiesel, have recently become popular, little is still known about the genotoxicity of emissions from these fuels. We analysed DNA damage expressed as the frequency of micronuclei (MN) in human bronchial epithelial cells (BEAS-2B), induced by extractable organic matter (EOM, tested concentrations: 1, 10 and 25 mu g/ml) obtained from particle emissions from various blends of biodiesel with diesel fuels (including neat diesel fuel (B0), a blend of 70% B0 and 30% biodiesel (B30) and neat biodiesel (B100)). We also tested the effect of selected diesel exhaust organic/genotoxic components [benzo[a] pyrene (B[a] P) concentrations: 25, 100 and 200 mu M, 1-nitropyrene (1-NP) concentrations: 1, 5 and 10 mu M, 3-nitrobenzanthrone (3-NBA) concentrations: 1, 5 and 50 mu M]. The cells were treated with the compounds for 28 and 48 hr. Our results showed that most of the tested compounds (except for the 25 mu M B[a] P, 28-hr treatment) significantly increased MN frequency. The genotoxicity of EOMs from the engine emissions of diesel and biodiesel engines was comparable. Both nitro-PAH compounds demonstrated higher genotoxic potential in comparison with B[a] P. Considering our results and due to increasing popularity of alternative fuels, it is prudent that the potential genotoxic effects of various fuels are investigated across engine technologies and operating conditions in a relevant model system.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30304 - Public and environmental health
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Basic & Clinical Pharmacology & Toxicology
ISSN
1742-7835
e-ISSN
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Volume of the periodical
121
Issue of the periodical within the volume
SI
Country of publishing house
US - UNITED STATES
Number of pages
7
Pages from-to
102-108
UT code for WoS article
000411036200012
EID of the result in the Scopus database
2-s2.0-85008413077