Pax2-Islet1 Transgenic Mice Are Hyperactive and Have Altered Cerebellar Foliation
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F17%3A00474451" target="_blank" >RIV/68378041:_____/17:00474451 - isvavai.cz</a>
Alternative codes found
RIV/86652036:_____/17:00474451 RIV/67985823:_____/17:00474451
Result on the web
<a href="http://dx.doi.org/10.1007/s12035-016-9716-6" target="_blank" >http://dx.doi.org/10.1007/s12035-016-9716-6</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s12035-016-9716-6" target="_blank" >10.1007/s12035-016-9716-6</a>
Alternative languages
Result language
angličtina
Original language name
Pax2-Islet1 Transgenic Mice Are Hyperactive and Have Altered Cerebellar Foliation
Original language description
The programming of cell fate by transcription factors requires precise regulation of their time and level of expression. The LIM-homeodomain transcription factor Islet1 (Isl1) is involved in cell-fate specification of motor neurons, and it may play a similar role in the inner ear. In order to study its role in the regulation of vestibulo-motor development, we investigated a transgenic mouse expressing Isl1 under the Pax2 promoter control (Tg (+/-) ). The transgenic mice show altered level, time, and place of expression of Isl1 but are viable. However, Tg (+/-) mice exhibit hyperactivity, including circling behavior, and progressive age-related decline in hearing, which has been reported previously. Here, we describe the molecular and morphological changes in the cerebellum and vestibular system that may cause the hyperactivity of Tg (+/-) mice. The transgene altered the formation of folia in the cerebellum, the distribution of calretinin labeled unipolar brush cells, and reduced the size of the cerebellum, inferior colliculus, and saccule. Age-related progressive reduction of calbindin expression was detected in Purkinje cells in the transgenic cerebella. The hyperactivity of Tg (+/-) mice is reduced upon the administration of picrotoxin, a non-competitive channel blocker for the gamma-aminobutyric acid (GABA) receptor chloride channels. This suggests that the overexpression of Isl1 significantly affects the functions of GABAergic neurons. We demonstrate that the overexpression of Isl1 affects the development and function of the cerebello-vestibular system, resulting in hyperactivity.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10605 - Developmental biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Molecular Neurobiology
ISSN
0893-7648
e-ISSN
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Volume of the periodical
54
Issue of the periodical within the volume
2
Country of publishing house
US - UNITED STATES
Number of pages
17
Pages from-to
1352-1368
UT code for WoS article
000394964100046
EID of the result in the Scopus database
2-s2.0-84957574582